UNIT 13.15 S. pombe Strain Maintenance and Media

  1. Susan L. Forsburg

Published Online: 1 NOV 2003

DOI: 10.1002/0471142727.mb1315s64

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Forsburg, S. L. 2003. S. pombe Strain Maintenance and Media. Current Protocols in Molecular Biology. 64:V:13.15:13.15.1–13.15.5.

Author Information

  1. Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California

Publication History

  1. Published Online: 1 NOV 2003
  2. Published Print: OCT 2003


Fission yeast can be grown and maintained using similar culture methods to those employed for budding yeast. The optimal media for S. pombe is somewhat different than that employed for S. cerevisiae, although budding yeast media can be used if necessary. Good sterile technique is essential to prevent contamination by airborne molds or bacteria.