UNIT 13.15 S. pombe Strain Maintenance and Media
Published Online: 1 NOV 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Forsburg, S. L. 2003. S. pombe Strain Maintenance and Media. Current Protocols in Molecular Biology. 64:V:13.15:13.15.1–13.15.5.
- Published Online: 1 NOV 2003
- Published Print: OCT 2003
Fission yeast can be grown and maintained using similar culture methods to those employed for budding yeast. The optimal media for S. pombe is somewhat different than that employed for S. cerevisiae, although budding yeast media can be used if necessary. Good sterile technique is essential to prevent contamination by airborne molds or bacteria.