UNIT 13.16 Growth and Manipulation of S. pombe
Published Online: 1 NOV 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Forsburg, S. L. 2003. Growth and Manipulation of S. pombe. Current Protocols in Molecular Biology. 64:V:13.16:13.16.1–13.16.17.
- Published Online: 1 NOV 2003
- Published Print: OCT 2003
This unit presents aspects specific to genetic and cytological manipulation of fission yeast, including mating type testing, crosses, preparingmaking diploids, and analysis of meiotic products, and basic methods of cell cycle synchronization and analysis. These methods are different from those used in budding yeast because they depend upon the distinct biology of S. pombe, particularly its unwillingness to be a diploid in normal growth conditions. Similarly, the different cell shape and growth behavior of S. pombe require different approaches to basic cell cycle analysis.