Unit

UNIT 13.16 Growth and Manipulation of S. pombe

  1. Susan L. Forsburg

Published Online: 1 NOV 2003

DOI: 10.1002/0471142727.mb1316s64

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Forsburg, S. L. 2003. Growth and Manipulation of S. pombe. Current Protocols in Molecular Biology. 64:V:13.16:13.16.1–13.16.17.

Author Information

  1. The Salk Institute for Biological Studies, La Jolla, California

Publication History

  1. Published Online: 1 NOV 2003
  2. Published Print: OCT 2003

Abstract

This unit presents aspects specific to genetic and cytological manipulation of fission yeast, including mating type testing, crosses, preparingmaking diploids, and analysis of meiotic products, and basic methods of cell cycle synchronization and analysis. These methods are different from those used in budding yeast because they depend upon the distinct biology of S. pombe, particularly its unwillingness to be a diploid in normal growth conditions. Similarly, the different cell shape and growth behavior of S. pombe require different approaches to basic cell cycle analysis.