UNIT 13.17 Introduction of DNA into S. pombe Cells
Published Online: 1 NOV 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Forsburg, S. L. 2003. Introduction of DNA into S. pombe Cells. Current Protocols in Molecular Biology. 64:V:13.17:13.17.1–13.17.8.
- Published Online: 1 NOV 2003
- Published Print: OCT 2003
Methods of transformation rely upon conditioning cells to take up DNA, and growing them under selective conditions to establish and maintain the plasmid or integration. Different methods may be used, including electroporation, treatment with lithium cations, or protoplast treatment which removes the cell wall. Protoplasts prepared as for transformation can also be induced to fuse with each other and undergo karyogamy, which provides a means of mating sterile strains.