Unit

UNIT 13.17 Introduction of DNA into S. pombe Cells

  1. Susan L. Forsburg

Published Online: 1 NOV 2003

DOI: 10.1002/0471142727.mb1317s64

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Forsburg, S. L. 2003. Introduction of DNA into S. pombe Cells. Current Protocols in Molecular Biology. 64:V:13.17:13.17.1–13.17.8.

Author Information

  1. The Salk Institute for Biological Studies, La Jolla, California

Publication History

  1. Published Online: 1 NOV 2003
  2. Published Print: OCT 2003

Abstract

Methods of transformation rely upon conditioning cells to take up DNA, and growing them under selective conditions to establish and maintain the plasmid or integration. Different methods may be used, including electroporation, treatment with lithium cations, or protoplast treatment which removes the cell wall. Protoplasts prepared as for transformation can also be induced to fuse with each other and undergo karyogamy, which provides a means of mating sterile strains.