UNIT 14.4 Detection of Hybridized Probe
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Rogers, M. 2001. Detection of Hybridized Probe. Current Protocols in Molecular Biology. 7:14.4:14.4.1–14.4.3.
- Published Online: 1 MAY 2001
- Published Print: JUL 1989
Autoradiography is used to detect and quantitate radioactive probe hybridized to cytological preparations. Autoradiographic film is used to detect 32P- or 35S-labeled probe, and can be useful in experiments dealing with large organs or tissues. Emulsion autoradiography is required to obtain resolution at the level of a single cell. Both procedures are described here.