Unit

UNIT 14.4 Detection of Hybridized Probe

  1. Melissa Rogers

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1404s07

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Rogers, M. 2001. Detection of Hybridized Probe. Current Protocols in Molecular Biology. 7:14.4:14.4.1–14.4.3.

Author Information

  1. Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1989

Abstract

Autoradiography is used to detect and quantitate radioactive probe hybridized to cytological preparations. Autoradiographic film is used to detect 32P- or 35S-labeled probe, and can be useful in experiments dealing with large organs or tissues. Emulsion autoradiography is required to obtain resolution at the level of a single cell. Both procedures are described here.