Unit

UNIT 14.5 Counterstaining and Mounting of Autoradiographed In Situ Hybridization Slides

  1. Rolf Zeller1,
  2. Melissa Rogers2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1405s24

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Zeller, R. and Rogers, M. 2001. Counterstaining and Mounting of Autoradiographed In Situ Hybridization Slides. Current Protocols in Molecular Biology. 24:14.5:14.5.1–14.5.5.

Author Information

  1. 1

    European Molecular Biology Laboratories, Heidelberg, Germany

  2. 2

    Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1993

Abstract

The morphology of specimen sections and the identity of specific areas are defined by lightly counterstaining sections. After staining, slides are dehydrated, mounted with coverslips, hardened, and cleaned for examination under the microscope. In the protocols provided in this unit, Giemsa stains predominantly the nuclei, hematoxylin/eosin stain differentiates both the nuclei and cytoplasm, toluidine blue staining is a simpler procedure that lightly stains both nuclei and cytoplasm, and Hoechst staining of nuclei provides a fast, easy, and effective way to simultaneously view the entire tissue and the regions of hybridization.