UNIT 14.5 Counterstaining and Mounting of Autoradiographed In Situ Hybridization Slides
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Zeller, R. and Rogers, M. 2001. Counterstaining and Mounting of Autoradiographed In Situ Hybridization Slides. Current Protocols in Molecular Biology. 24:14.5.1–14.5.5.
- Published Online: 1 MAY 2001
- Published Print: OCT 1993
The morphology of specimen sections and the identity of specific areas are defined by lightly counterstaining sections. After staining, slides are dehydrated, mounted with coverslips, hardened, and cleaned for examination under the microscope. In the protocols provided in this unit, Giemsa stains predominantly the nuclei, hematoxylin/eosin stain differentiates both the nuclei and cytoplasm, toluidine blue staining is a simpler procedure that lightly stains both nuclei and cytoplasm, and Hoechst staining of nuclei provides a fast, easy, and effective way to simultaneously view the entire tissue and the regions of hybridization.