Unit

UNIT 14.6 Immunohistochemistry

  1. Marsha Goldstein1,
  2. Simon Watkins2

Published Online: 1 JAN 2008

DOI: 10.1002/0471142727.mb1406s81

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Goldstein, M. and Watkins, S. 2008. Immunohistochemistry. Current Protocols in Molecular Biology. 81:14.6:14.6.1–14.6.23.

Author Information

  1. 1

    Bio-Reference Laboratories, Inc., Elmwood Park, New Jersey

  2. 2

    Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts

Publication History

  1. Published Online: 1 JAN 2008
  2. Published Print: JAN 2008

Abstract

Immunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright-field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided. Curr. Protoc. Mol. Biol. 81:14.6.1-14.6.23. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • protein localization;
  • immunohistochemistry;
  • immunofluorescence;
  • immunoperoxidase;
  • immunogold