Unit

UNIT 14.8 In Situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets

  1. Omar Bagasra

Published Online: 1 APR 2008

DOI: 10.1002/0471142727.mb1408s82

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Bagasra, O. 2008. In Situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets. Current Protocols in Molecular Biology. 82:14.8:14.8.1–14.8.28.

Author Information

  1. South Carolina Center for Biotechnology, Claflin University, Orangeburg, South Carolina

Publication History

  1. Published Online: 1 APR 2008
  2. Published Print: APR 2008

Abstract

This unit provides detailed methods and material descriptions for in situ hybridization following in situ amplification of DNA or RNA by PCR. It includes all essential components of the techniques, including variations suitable for different kinds of tissue and cell preparations. Planning, controls, and critical parameters for the amplification steps are discussed. Curr. Protoc. Mol. Biol. 82:14.8.1-14.8.28. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • in situ hybridization;
  • PCR;
  • RT-PCR;
  • probes;
  • primers;
  • amplification;
  • chambers