UNIT 14.8 In Situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets
Published Online: 1 APR 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Bagasra, O. 2008. In Situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets. Current Protocols in Molecular Biology. 82:14.8:14.8.1–14.8.28.
- Published Online: 1 APR 2008
- Published Print: APR 2008
This unit provides detailed methods and material descriptions for in situ hybridization following in situ amplification of DNA or RNA by PCR. It includes all essential components of the techniques, including variations suitable for different kinds of tissue and cell preparations. Planning, controls, and critical parameters for the amplification steps are discussed. Curr. Protoc. Mol. Biol. 82:14.8.1-14.8.28. © 2008 by John Wiley & Sons, Inc.
- in situ hybridization;