UNIT 14.9 Whole-Mount In Situ Hybridization and Detection of RNAs in Vertebrate Embryos and Isolated Organs
Published Online: 1 MAY 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Pizard, A., Haramis, A., Carrasco, A. E., Franco, P., López, S. and Paganelli, A. 2004. Whole-Mount In Situ Hybridization and Detection of RNAs in Vertebrate Embryos and Isolated Organs. Current Protocols in Molecular Biology. 66:14.9:14.9.1–14.9.24.
- Published Online: 1 MAY 2004
- Published Print: APR 2004
Nonisotopic in situ hybridization using intact embryos or organs is an important method for determining the spatial distribution of RNAs. Because it allows the analysis of large numbers of samples, it is amenable to temporal expression studies and comparison between different genotypes. It offers sensitivity and reproducibility. In addition, histological details are not lost during the staining process. The protocols in this unit can be used for whole-mount in situ hybridization in Xenopus, mouse, and chicken embryos, as well as dissected organs from mouse and chicken. Preparation of digoxigenin-labeled riboprobes is also described.
- in situ hybridization;
- gene expression;