UNIT 14.10 Principles and Application of Fluorescence Microscopy

  1. Donald Coling1,
  2. Bechara Kachar2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1410s44

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Coling, D. and Kachar, B. 2001. Principles and Application of Fluorescence Microscopy. Current Protocols in Molecular Biology. 44:14.10:14.10.1–14.10.11.

Author Information

  1. 1

    University of California Berkeley, Berkeley, California

  2. 2

    National Institute on Deafness and Other Communication Disorders, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


This overview discusses the principle of fluorescence along with practical discussions of fluorescent molecular probes, filters and filter sets, multiband filters and multi-dye fluorescence, light sources, objective lenses, image resolution and the point-spread function, fluorescence microscopy of living cells, and immunolabeling.