UNIT 14.11 Basic Confocal Microscopy
Published Online: 1 JAN 2008
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Smith, C. L. 2008. Basic Confocal Microscopy. Current Protocols in Molecular Biology. 81:14.11.1–14.11.18.
- Published Online: 1 JAN 2008
- Published Print: JAN 2008
This unit introduces the reader to the basic principles of confocal microscopy and the design and capabilities of current confocal microscopes. The advantages and disadvantages of confocal microscopy compared to other techniques for fluorescence imaging are described. There are also practical guidelines for sample preparation and optimization of imaging parameters, as well as examples of some of the applications of confocal microscopy. Curr. Protoc. Mol. Biol. 81:14.11.1-14.11.18. © 2008 by John Wiley & Sons, Inc.
Keywords: confocal microscopy; fluorescence; imaging; resolution; three-dimensional reconstruction