Unit

UNIT 14.13 Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis

  1. David Martin,
  2. Michael Lenardo

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1413s49

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Martin, D. and Lenardo, M. 2001. Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis. Current Protocols in Molecular Biology. 49:14.13.1–14.13.21.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2000

Abstract

As programmed cell death (PCD), or apoptosis, has emerged as an important regulator of development and homeostasis in multicellular organisms, methods to quantify apoptosis and to distinguish it from necrosis have been developed. Necrosis refers to the morphology usually associated with accidental cell death, while apoptosis is seen when cell death is programmed or physiologically regulated. This unit presents a set of assays for these purposes, many of which are technically very simple. Featured in this unit is the TUNEL method of detecting cells that exhibit DNA fragmentation, which can also be performed on tissue sections to locate apoptotic cells in situ.