UNIT 14.13 Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Martin, D. and Lenardo, M. 2001. Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis. Current Protocols in Molecular Biology. 49:14.13.1–14.13.21.
- Published Online: 1 MAY 2001
- Published Print: JAN 2000
As programmed cell death (PCD), or apoptosis, has emerged as an important regulator of development and homeostasis in multicellular organisms, methods to quantify apoptosis and to distinguish it from necrosis have been developed. Necrosis refers to the morphology usually associated with accidental cell death, while apoptosis is seen when cell death is programmed or physiologically regulated. This unit presents a set of assays for these purposes, many of which are technically very simple. Featured in this unit is the TUNEL method of detecting cells that exhibit DNA fragmentation, which can also be performed on tissue sections to locate apoptotic cells in situ.