UNIT 14.14 Whole-Mount Histochemical Detection of β-Galactosidase Activity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Pereira, F. A. 2001. Whole-Mount Histochemical Detection of β-Galactosidase Activity. Current Protocols in Molecular Biology. 50:14.14:14.14.1–14.14.8.
- Published Online: 1 MAY 2001
- Published Print: APR 2000
This unit describes fixation and staining for b-galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants. To help achieve consistent results with larger samples, an alternate protocol details preparation of thick sections. Support protocols are provided for clearing of tissues for better resolution and paraffin-embedding the whole embryo.