Unit

UNIT 14.14 Whole-Mount Histochemical Detection of β-Galactosidase Activity

  1. Fred A. Pereira

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1414s50

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Pereira, F. A. 2001. Whole-Mount Histochemical Detection of β-Galactosidase Activity. Current Protocols in Molecular Biology. 50:14.14:14.14.1–14.14.8.

Author Information

  1. Baylor College of Medicine, Houston, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000

Abstract

This unit describes fixation and staining for b-galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants. To help achieve consistent results with larger samples, an alternate protocol details preparation of thick sections. Support protocols are provided for clearing of tissues for better resolution and paraffin-embedding the whole embryo.