UNIT 14.15 Basic Image Analysis and Manipulation in ImageJ
Published Online: 1 APR 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Hartig, S. M. 2013. Basic Image Analysis and Manipulation in ImageJ. Current Protocols in Molecular Biology. 102:14.15.1–14.15.12.
- Published Online: 1 APR 2013
- Published Print: APR 2013
Image analysis methods have been developed to provide quantitative assessment of microscopy data. In this unit, basic aspects of image analysis are outlined, including software installation, data import, image processing functions, and analytical tools that can be used to extract information from microscopy data using ImageJ. Step-by-step protocols for analyzing objects in a fluorescence image and extracting information from two-color tissue images collected by bright-field microscopy are included. Curr. Protoc. Mol. Biol. 102:14.15.1–14.15.12. © 2013 by John Wiley & Sons, Inc.
- image analysis;
- object identification;
- spectral unmixing