Unit

UNIT 14.15 Basic Image Analysis and Manipulation in ImageJ

  1. Sean M. Hartig

Published Online: 1 APR 2013

DOI: 10.1002/0471142727.mb1415s102

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Hartig, S. M. 2013. Basic Image Analysis and Manipulation in ImageJ. Current Protocols in Molecular Biology. 102:14.15.1–14.15.12.

Author Information

  1. Baylor College of Medicine, Houston, Texas

Publication History

  1. Published Online: 1 APR 2013
  2. Published Print: APR 2013

Abstract

Image analysis methods have been developed to provide quantitative assessment of microscopy data. In this unit, basic aspects of image analysis are outlined, including software installation, data import, image processing functions, and analytical tools that can be used to extract information from microscopy data using ImageJ. Step-by-step protocols for analyzing objects in a fluorescence image and extracting information from two-color tissue images collected by bright-field microscopy are included. Curr. Protoc. Mol. Biol. 102:14.15.1–14.15.12. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • ImageJ;
  • microscopy;
  • image analysis;
  • object identification;
  • spectral unmixing