UNIT 14.16 Three-Dimensional Reconstruction of Tissues
Published Online: 1 MAY 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Cruz, F., Lee, R. T. and Huang, H. 2006. Three-Dimensional Reconstruction of Tissues. Current Protocols in Molecular Biology. 74:14.16:14.16.1–14.16.13.
- Published Online: 1 MAY 2006
- Published Print: APR 2006
Cellular morphology is inherently three-dimensional. However, most histological techniques for tissue analysis focus on extracting information from two-dimensional slices of fixed samples or dissociated cells. These techniques result in a significant loss of the three-dimensional information of the tissue, including true cell volume, orientation, and whole cell shape. This unit discusses various options for three-dimensional imaging, provides a protocol for performing post-processing reconstruction based on serial slicing, and discusses the current advantages and limitations of the three-dimensional approach to quantitative tissue analysis. The focus of this protocol is on cardiac tissue, but the techniques can be applied to any solid tissue.
- fluorescence microscopy;
- image registration;
- cell morphology