UNIT 14.20 Computer Control of Microscopes Using µManager

  1. Arthur Edelstein1,
  2. Nenad Amodaj2,
  3. Karl Hoover1,
  4. Ron Vale1,3,
  5. Nico Stuurman1,3

Published Online: 1 OCT 2010

DOI: 10.1002/0471142727.mb1420s92

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Edelstein, A., Amodaj, N., Hoover, K., Vale, R. and Stuurman, N. 2010. Computer Control of Microscopes Using µManager. Current Protocols in Molecular Biology. 92:14.20:14.20.1–14.20.17.

Author Information

  1. 1

    Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California

  2. 2

    100x Imaging, San Francisco, California

  3. 3

    Howard Hughes Medical Institute, Chevy Chase, Maryland

Publication History

  1. Published Online: 1 OCT 2010
  2. Published Print: OCT 2010


With the advent of digital cameras and motorization of mechanical components, computer control of microscopes has become increasingly important. Software for microscope image acquisition should not only be easy to use, but also enable and encourage novel approaches. The open-source software package µManager aims to fulfill those goals. This unit provides step-by-step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software. Curr. Protoc. Mol. Biol. 92:14.20.1-14.20.17. © 2010 by John Wiley & Sons, Inc.


  • Micro-Manager;
  • µManager;
  • microscopy;
  • automation;
  • open source;
  • cross platform;
  • ImageJ;
  • digital imaging;
  • fluorescence microscopy;
  • multi-dimensional acquisition