Unit

UNIT 14.23 Visualizing Genomes with Oligopaint FISH Probes

  1. Brian J. Beliveau1,
  2. Nicholas Apostolopoulos2,
  3. Chao-ting Wu1

Published Online: 6 JAN 2014

DOI: 10.1002/0471142727.mb1423s105

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Beliveau, B. J., Apostolopoulos, N. and Wu, C.-t. 2014. Visualizing Genomes with Oligopaint FISH Probes. Current Protocols in Molecular Biology. 105:14.23:14.23.1–14.23.20.

Author Information

  1. 1

    Department of Genetics, Harvard Medical School, Boston, Massachusetts

  2. 2

    Yale School of Medicine, New Haven, Connecticut

Publication History

  1. Published Online: 6 JAN 2014

Abstract

Oligopaint probes are fluorescently labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D-FISH, and sample preparation. Curr. Protoc. Mol. Biol. 105:14.23.1-14.23.20. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • Oligopaint;
  • oligonucleotide;
  • complex DNA library;
  • FISH