Unit

UNIT 15.4 Molecular Cloning of PCR Products

  1. Michael Finney1,
  2. Paul E. Nisson2,
  3. Ayoub Rashtchian (UDG cloning)2

Published Online: 1 NOV 2001

DOI: 10.1002/0471142727.mb1504s56

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Finney, M., Nisson, P. E. and Rashtchian, A. 2001. Molecular Cloning of PCR Products. Current Protocols in Molecular Biology. 56:15.4:15.4.1–15.4.11.

Author Information

  1. 1

    MJ Research, Watertown, Massachusetts

  2. 2

    Life Technologies, Inc., Gaithersburg, Maryland

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: OCT 2001

Abstract

It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high-quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This unit describes the strategies for generating and manipulating suitable ends on the PCR fragments.