UNIT 15.4 Molecular Cloning of PCR Products
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Finney, M., Nisson, P. E. and Rashtchian, A. 2001. Molecular Cloning of PCR Products. Current Protocols in Molecular Biology. 56:15.4:15.4.1–15.4.11.
- Published Online: 1 NOV 2001
- Published Print: OCT 2001
It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high-quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This unit describes the strategies for generating and manipulating suitable ends on the PCR fragments.