Unit

UNIT 15.5 Enzymatic Amplification of RNA by PCR (RT-PCR)

  1. Stephen M. Beverly

Published Online: 1 NOV 2001

DOI: 10.1002/0471142727.mb1505s56

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Beverly, S. M. 2001. Enzymatic Amplification of RNA by PCR (RT-PCR). Current Protocols in Molecular Biology. 56:15.5:15.5.1–15.5.6.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: OCT 2001

Abstract

Methods for enzymatic amplification of RNA by the polymerase chain reaction (RT-PCR) are highlighted in this unit. The Basic Protocol is especially useful for rare RNAs because all steps (annealing, reverse transcription, and amplification) are performed under optimal conditions, thereby maximizing efficiency and recovery. These are also important considerations when amplifying heterogeneous RNA populations or large RNAs. Although there are many commercially available kits for this procedure, the protocols listed here are good solid protocols that will work and that have withstood the test of time.