UNIT 15.5 Enzymatic Amplification of RNA by PCR (RT-PCR)
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Beverly, S. M. 2001. Enzymatic Amplification of RNA by PCR (RT-PCR). Current Protocols in Molecular Biology. 56:15.5:15.5.1–15.5.6.
- Published Online: 1 NOV 2001
- Published Print: OCT 2001
Methods for enzymatic amplification of RNA by the polymerase chain reaction (RT-PCR) are highlighted in this unit. The Basic Protocol is especially useful for rare RNAs because all steps (annealing, reverse transcription, and amplification) are performed under optimal conditions, thereby maximizing efficiency and recovery. These are also important considerations when amplifying heterogeneous RNA populations or large RNAs. Although there are many commercially available kits for this procedure, the protocols listed here are good solid protocols that will work and that have withstood the test of time.