UNIT 15.6 cDNA Amplification Using One-Sided (Anchored) PCR
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Dorit, R. L. and Ohara, O. 2001. cDNA Amplification Using One-Sided (Anchored) PCR. Current Protocols in Molecular Biology. 17:15.6:15.6.1–15.6.10.
- Published Online: 1 MAY 2001
- Published Print: JAN 1992
The polymerase chain reaction (PCR) offers an opportunity to directly select, amplify, and isolate a message of interest. This unit describes anchored PCR, a modification of the standard reaction that allows amplification of full-length mRNA when only a small amount of sequence information is available. Anchored PCR can be employed when only a small region of sequence lying within the mRNA is known in advance, unlike the amplification of RNA or cDNA by conventional PCR which requires prior knowledge of the sequences flanking the region of interest to design the PCR primers.