Unit

UNIT 15.6 cDNA Amplification Using One-Sided (Anchored) PCR

  1. Robert L. Dorit1,
  2. Osamu Ohara2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1506s17

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Dorit, R. L. and Ohara, O. 2001. cDNA Amplification Using One-Sided (Anchored) PCR. Current Protocols in Molecular Biology. 17:15.6:15.6.1–15.6.10.

Author Information

  1. 1

    Yale University, New Haven, Connecticut

  2. 2

    Shionogi Research Laboratories, Osaka, Japan

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1992

Abstract

The polymerase chain reaction (PCR) offers an opportunity to directly select, amplify, and isolate a message of interest. This unit describes anchored PCR, a modification of the standard reaction that allows amplification of full-length mRNA when only a small amount of sequence information is available. Anchored PCR can be employed when only a small region of sequence lying within the mRNA is known in advance, unlike the amplification of RNA or cDNA by conventional PCR which requires prior knowledge of the sequences flanking the region of interest to design the PCR primers.