Unit

UNIT 15.7 Quantitation of Rare DNAs by PCR

  1. Donald M. Coen

Published Online: 1 NOV 2001

DOI: 10.1002/0471142727.mb1507s56

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Coen, D. M. 2001. Quantitation of Rare DNAs by PCR. Current Protocols in Molecular Biology. 56:15.7:15.7.1–15.7.8.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: OCT 2001

Abstract

This unit presents a protocol that uses the polymerase chain reaction (PCR) to quantitate the numbers of a particular DNA sequence, from 1 to 20,000 molecules per sample. In addition, it helps assess the presence of contaminating sequences, which can seriously affect the outcome of the procedure.