UNIT 15.7 Quantitation of Rare DNAs by PCR
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Coen, D. M. 2001. Quantitation of Rare DNAs by PCR. Current Protocols in Molecular Biology. 56:15.7.1–15.7.8.
- Published Online: 1 NOV 2001
- Published Print: OCT 2001
This unit presents a protocol that uses the polymerase chain reaction (PCR) to quantitate the numbers of a particular DNA sequence, from 1 to 20,000 molecules per sample. In addition, it helps assess the presence of contaminating sequences, which can seriously affect the outcome of the procedure.