Unit

UNIT 15.12 Library Generation by Gene Shuffling

  1. Adam J. Meyer,
  2. Jared W. Ellefson,
  3. Andrew D. Ellington

Published Online: 6 JAN 2014

DOI: 10.1002/0471142727.mb1512s105

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Meyer, A. J., Ellefson, J. W. and Ellington, A. D. 2014. Library Generation by Gene Shuffling. Current Protocols in Molecular Biology. 105:15.12:15.12.1–15.12.7.

Author Information

  1. University of Texas at Austin, Austin, Texas

Publication History

  1. Published Online: 6 JAN 2014

Abstract

This unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer-less PCR. The resulting chimeric genes can then be screened or selected for a desired function. Curr. Protoc. Mol. Biol. 105:15.12.1-15.12.7. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • directed evolution;
  • recombination;
  • PCR