UNIT 15.12 Library Generation by Gene Shuffling
Published Online: 6 JAN 2014
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Meyer, A. J., Ellefson, J. W. and Ellington, A. D. 2014. Library Generation by Gene Shuffling. Current Protocols in Molecular Biology. 105:15.12:15.12.1–15.12.7.
- Published Online: 6 JAN 2014
This unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer-less PCR. The resulting chimeric genes can then be screened or selected for a desired function. Curr. Protoc. Mol. Biol. 105:15.12.1-15.12.7. © 2014 by John Wiley & Sons, Inc.
- directed evolution;