Unit

UNIT 16.4B Enzymatic and Chemical Cleavage of Fusion Proteins

  1. Edward R. LaVallie1,
  2. John M. McCoy1,
  3. Donald B. Smith (thrombin cleavage)2,
  4. Paul Riggs (denaturing fusion proteins)3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1604bs28

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

LaVallie, E. R., McCoy, J. M., Smith, D. B. and Riggs, P. 2001. Enzymatic and Chemical Cleavage of Fusion Proteins. Current Protocols in Molecular Biology. 28:I:16.4B:16.4.5-16–16.4.17.

Author Information

  1. 1

    Genetic Institute, Cambridge, Massachusetts

  2. 2

    University of Edinburg, Edinburg, Scotland

  3. 3

    New England Biolabs, Beverly, Masssachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1994

Abstract

This unit provides protocols for some commonly used methods of site-specific cleavage of fusion proteins. The first three protocols describe enzymatic cleavage of proteins using proteases (factor Xa, thrombin, and enterokinase) that display highly restricted specificities, which greatly decrease the likelihood that unwanted secondary cuts will occur. Three additional protocols describe specific cleavage of fusion proteins with chemical reagents (cyanogen bromide, hydroxylamine, and low pH) as an alternative to enzymatic cleavage.