UNIT 16.4B Enzymatic and Chemical Cleavage of Fusion Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
LaVallie, E. R., McCoy, J. M., Smith, D. B. and Riggs, P. 2001. Enzymatic and Chemical Cleavage of Fusion Proteins. Current Protocols in Molecular Biology. 28:I:16.4B:16.4.5-16–16.4.17.
- Published Online: 1 MAY 2001
- Published Print: OCT 1994
This unit provides protocols for some commonly used methods of site-specific cleavage of fusion proteins. The first three protocols describe enzymatic cleavage of proteins using proteases (factor Xa, thrombin, and enterokinase) that display highly restricted specificities, which greatly decrease the likelihood that unwanted secondary cuts will occur. Three additional protocols describe specific cleavage of fusion proteins with chemical reagents (cyanogen bromide, hydroxylamine, and low pH) as an alternative to enzymatic cleavage.