Unit

UNIT 16.9 Overview of the Baculovirus Expression System

  1. Cheryl Isaac Murphy1,
  2. Helen Piwnica-Worms2,
  3. Stefan Grünwald3,
  4. William G. Romanow4,
  5. Nicole Francis5,
  6. Hua-Ying Fan5

Published Online: 1 FEB 2004

DOI: 10.1002/0471142727.mb1609s65

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Murphy, C. I., Piwnica-Worms, H., Grünwald, S., Romanow, W. G., Francis, N. and Fan, H.-Y. 2004. Overview of the Baculovirus Expression System. Current Protocols in Molecular Biology. 65:II:16.9:16.9.1–16.9.10.

Author Information

  1. 1

    Aquila Biopharmaceuticals, Worcester, Massachusetts

  2. 2

    Washington University School of Medicine, St. Louis, Missouri

  3. 3

    Pharmingen, San Diego, California

  4. 4

    Corning Costar, Portsmouth, New Hampshire

  5. 5

    Massachusetts General Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: JAN 2004

Abstract

Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This unit gives an overview of the baculovirus expression system, including discussion of the baculovirus life cycle, and post-translational modifications that occur in insect cells. In addition, the steps for overproducing proteins in the baculovirus systems are described along with recommendations for choosing an appropriate baculovirus vector and DNA, and reagents and equipment necessary for implementing the whole overexpression system.