UNIT 16.11 Expression and Purification of Recombinant Proteins Using the Baculovirus System
Published Online: 1 FEB 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Murphy, C. I., Piwnica-Worms, H., Grünwald, S., Romanow, W. G., Francis, N. and Fan, H.-Y. 2004. Expression and Purification of Recombinant Proteins Using the Baculovirus System. Current Protocols in Molecular Biology. 65:II:16.11:16.11.1–16.11.14.
- Published Online: 1 FEB 2004
- Published Print: JAN 2004
This unit describes how to analyze protein expression in cells infected with recombinant baculovirus on a small scale for optimizing protein production , how to maximize and scale up recombinant protein production, and how to purify recombinant proteins.