Unit

UNIT 16.11 Expression and Purification of Recombinant Proteins Using the Baculovirus System

  1. Cheryl Isaac Murphy1,
  2. Helen Piwnica-Worms2,
  3. Stefan Grünwald3,
  4. William G. Romanow4,
  5. Nicole Francis5,
  6. Hua-Ying Fan5

Published Online: 1 FEB 2004

DOI: 10.1002/0471142727.mb1611s65

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Murphy, C. I., Piwnica-Worms, H., Grünwald, S., Romanow, W. G., Francis, N. and Fan, H.-Y. 2004. Expression and Purification of Recombinant Proteins Using the Baculovirus System. Current Protocols in Molecular Biology. 65:II:16.11:16.11.1–16.11.14.

Author Information

  1. 1

    Aquila Biopharmaceuticals, Worcester, Massachusetts

  2. 2

    Washington University School of Medicine, St. Louis, Missouri

  3. 3

    Pharmingen, San Diego, California

  4. 4

    Corning Costar, Portsmouth, New Hampshire

  5. 5

    Massachusetts General Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: JAN 2004

Abstract

This unit describes how to analyze protein expression in cells infected with recombinant baculovirus on a small scale for optimizing protein production , how to maximize and scale up recombinant protein production, and how to purify recombinant proteins.