Unit

UNIT 16.16 Preparation of Cell Cultures and Vaccinia Virus Stocks

  1. Patricia L. Earl1,
  2. Norman Cooper1,
  3. Linda S. Wyatt1,
  4. Bernard Moss1,
  5. Miles W. Carroll2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1616s43

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Earl, P. L., Cooper, N., Wyatt, L. S., Moss, B. and Carroll, M. W. 2001. Preparation of Cell Cultures and Vaccinia Virus Stocks. Current Protocols in Molecular Biology. 43:III:16.16:16.16.1–16.16.13.

Author Information

  1. 1

    National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland

  2. 2

    Oxford BioMedica, Oxford, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1998

Abstract

This unit describes the maintenance of cell lines used with vaccinia virus, both in monolayer cultures and in suspension. The suspended cell culture is then used in the preparation of vaccinia virus stocks. The preparation of chick embryo fibroblasts (CEF) is also presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Additionally, support protocols are presented for the titration of standard and MVA vaccinia virus stocks.