Unit

UNIT 16.17 Generation of Recombinant Vaccinia Viruses

  1. Patricia L. Earl1,
  2. Bernard Moss1,
  3. Linda S. Wyatt1,
  4. Miles W. Carroll2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1617s43

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Earl, P. L., Moss, B., Wyatt, L. S. and Carroll, M. W. 2001. Generation of Recombinant Vaccinia Viruses. Current Protocols in Molecular Biology. 43:III:16.17:16.17.1–16.17.19.

Author Information

  1. 1

    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

  2. 2

    Oxford BioMedica, Oxford, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1998

Abstract

This unit first describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector to generate a recombinant virus. Methods are also presented for purifying vaccinia virus and for isolating viral DNA, which can be used during transfection. Also presented are selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented.