UNIT 16.21 Overview of the HIV-1 Lentiviral Vector System
Published Online: 1 NOV 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Ramezani, A. and Hawley, R. G. 2002. Overview of the HIV-1 Lentiviral Vector System. Current Protocols in Molecular Biology. 60:III:16.21:16.21.1–16.21.15.
- Published Online: 1 NOV 2002
- Published Print: OCT 2002
Replication-defective oncoretroviral vectors have been the most widely used vehicles for gene-transfer studies because of their capacity to efficiently introduce and stably express transgenes in mammalian cells. A limitation of oncoretroviral vectors is that cell division is required for proviral integration into the host genome. By comparison, lentiviruses such as human immunodeficiency virus type 1 (HIV-1) have evolved a nuclear-import machinery that allows them to infect nondividing as well as dividing cells. This unique property has led to the development of lentiviral vectors for gene delivery to a variety of nondividing or slowly dividing cells including neurons and glial cells of the central nervous system and others. This unit is intended to provide an overview of HIV-1 molecular biology and an introduction to successive generations of HIV-1-based lentiviral vectors.