Unit

UNIT 16.22 Generation of HIV-1-Based Lentiviral Vector Particles

  1. Ali Ramezani1,
  2. Robert G. Hawley1,2

Published Online: 1 NOV 2002

DOI: 10.1002/0471142727.mb1622s60

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Ramezani, A. and Hawley, R. G. 2002. Generation of HIV-1-Based Lentiviral Vector Particles. Current Protocols in Molecular Biology. 60:III:16.22:16.22.1–16.22.15.

Author Information

  1. 1

    American Red Cross, Rockville, Maryland

  2. 2

    The George washington University, Washington, D.C.

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: OCT 2002

Abstract

This unit presents protocols outlining the methodology and techniques involved in the construction and application of HIV-1-based lentiviral vector systems. Also described are procedures that can be used to concentrate and purify high-titer recombinant lentiviral vector preparations, as well as protocols for transduction of adherent and suspension cells.