UNIT 16.22 Generation of HIV-1-Based Lentiviral Vector Particles
Published Online: 1 NOV 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Ramezani, A. and Hawley, R. G. 2002. Generation of HIV-1-Based Lentiviral Vector Particles. Current Protocols in Molecular Biology. 60:III:16.22:16.22.1–16.22.15.
- Published Online: 1 NOV 2002
- Published Print: OCT 2002
This unit presents protocols outlining the methodology and techniques involved in the construction and application of HIV-1-based lentiviral vector systems. Also described are procedures that can be used to concentrate and purify high-titer recombinant lentiviral vector preparations, as well as protocols for transduction of adherent and suspension cells.