UNIT 16.24 Helper-Dependent Adenoviral Vectors
Published Online: 1 FEB 2005
Copyright © 2005 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Oka, K. and Chan, L. 2005. Helper-Dependent Adenoviral Vectors. Current Protocols in Molecular Biology. 69:III:16.24:16.24.1–16.24.23.
- Published Online: 1 FEB 2005
- Published Print: JAN 2005
The helper-dependent adenovirus (HDAd) is a recently developed adenovirus-based vector with an improved safety profile and long-term transgene expression. In this unit, a basic procedure for HDAd production using the Cre-loxP system is presented. Amplification and large-scale production of the vector can be done in both adherent and suspension cell culture systems. Included are protocols for Southern blot analysis to monitor vector amplification, slot blot assay to determine the infectious titer of the purified HDAd, and real-time PCR to detect helper virus contamination in the preparation.
- Helper-dependent adenoviral vector;
- helper virus;
- adherent cells;
- suspension cells