Unit

UNIT 16.24 Helper-Dependent Adenoviral Vectors

  1. Kazuhiro Oka,
  2. Lawrence Chan

Published Online: 1 FEB 2005

DOI: 10.1002/0471142727.mb1624s69

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Oka, K. and Chan, L. 2005. Helper-Dependent Adenoviral Vectors. Current Protocols in Molecular Biology. 69:III:16.24:16.24.1–16.24.23.

Author Information

  1. Baylor College of Medicine, Houston, Texas

Publication History

  1. Published Online: 1 FEB 2005
  2. Published Print: JAN 2005

Abstract

The helper-dependent adenovirus (HDAd) is a recently developed adenovirus-based vector with an improved safety profile and long-term transgene expression. In this unit, a basic procedure for HDAd production using the Cre-loxP system is presented. Amplification and large-scale production of the vector can be done in both adherent and suspension cell culture systems. Included are protocols for Southern blot analysis to monitor vector amplification, slot blot assay to determine the infectious titer of the purified HDAd, and real-time PCR to detect helper virus contamination in the preparation.

Keywords:

  • Helper-dependent adenoviral vector;
  • helper virus;
  • adherent cells;
  • suspension cells