Unit

UNIT 16.25 Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use

  1. Vivian W. Choi,
  2. Aravind Asokan,
  3. Rebecca A. Haberman,
  4. Richard Jude Samulski

Published Online: 1 APR 2007

DOI: 10.1002/0471142727.mb1625s78

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Choi, V. W., Asokan, A., Haberman, R. A. and Samulski, R. J. 2007. Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use. Current Protocols in Molecular Biology. 78:III:16.25:16.25.1–16.25.24.

Author Information

  1. University of North Carolina, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 APR 2007
  2. Published Print: APR 2007

Abstract

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection in many different cell types and its ability to persist and lead to long-term gene expression. The vector is also a valuable tool in molecular biology experiments. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free, recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors.

Keywords:

  • adeno-associated virus;
  • gene delivery;
  • purification;
  • serotypes;
  • transfection;
  • viral vectors