UNIT 16.25 Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use
Published Online: 1 APR 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Choi, V. W., Asokan, A., Haberman, R. A. and Samulski, R. J. 2007. Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use. Current Protocols in Molecular Biology. 78:III:16.25:16.25.1–16.25.24.
- Published Online: 1 APR 2007
- Published Print: APR 2007
Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection in many different cell types and its ability to persist and lead to long-term gene expression. The vector is also a valuable tool in molecular biology experiments. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free, recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors.
- adeno-associated virus;
- gene delivery;
- viral vectors