Preparation and Analysis of Glycoconjugates
Published Online: 1 OCT 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Varki, A., Freeze, H. H. and Manzi, A. E. 2009. Preparation and Analysis of Glycoconjugates. Current Protocols in Molecular Biology. 88:17.0.1–17.0.12.
- Published Online: 1 OCT 2009
- Published Print: OCT 2009
Whereas DNA, RNA, and proteins are linear polymers that can usually be directly sequenced, glycans show substantially more complexity, having branching and anomeric configurations (α and β linkages). The biosynthesis of glycans, termed glycosylation, is extremely complex, is not template-driven, varies among different cell types, and cannot be easily predicted from simple rules. This overview discusses the stereochemistry of mono- and oligosaccharides and provides diagrammatic representations of monosaccharides (Fisher projections and Haworth representations) and formulas for representation of glycan chains. A glossary of terms used in glycobiology is also provided. Curr. Protoc. Mol. Biol. 88:17.0.1-17.0.12. © 2009 by John Wiley & Sons, Inc.
- glycan analysis;
- sugar symbols;