Chapter Introduction

Preparation and Analysis of Glycoconjugates

  1. Ajit Varki1,
  2. Hudson H. Freeze2,
  3. Adriana E. Manzi1

Published Online: 1 OCT 2009

DOI: 10.1002/0471142727.mb1700s88

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Varki, A., Freeze, H. H. and Manzi, A. E. 2009. Preparation and Analysis of Glycoconjugates. Current Protocols in Molecular Biology. 88:17.0:17.0.1–17.0.12.

Author Information

  1. 1

    University of California San Diego, La Jolla, California

  2. 2

    Sanford Children's Health Research Center and Burnham Institute for Medical Research, La Jolla, California

Publication History

  1. Published Online: 1 OCT 2009
  2. Published Print: OCT 2009

Abstract

Whereas DNA, RNA, and proteins are linear polymers that can usually be directly sequenced, glycans show substantially more complexity, having branching and anomeric configurations (α and β linkages). The biosynthesis of glycans, termed glycosylation, is extremely complex, is not template-driven, varies among different cell types, and cannot be easily predicted from simple rules. This overview discusses the stereochemistry of mono- and oligosaccharides and provides diagrammatic representations of monosaccharides (Fisher projections and Haworth representations) and formulas for representation of glycan chains. A glossary of terms used in glycobiology is also provided. Curr. Protoc. Mol. Biol. 88:17.0.1-17.0.12. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • glycan;
  • monosaccharide;
  • glycan analysis;
  • sugar symbols;
  • glycoconjugate;
  • glycobiology