UNIT 17.3 Special Considerations for Glycolipids and Their Purification
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Smith, D. F. and Prieto, P. A. 2001. Special Considerations for Glycolipids and Their Purification. Current Protocols in Molecular Biology. 22:I:17.3:17.3.1–17.3.13.
- Published Online: 1 MAY 2001
- Published Print: APR 1993
This unit describes the antigenic stimulation of in vitro antibody production by B cells and the subsequent measurement of secreted antibodies. A generalized system for inducing in vitro antibody production is presented along with a procedure for quantifying the number of antibody-producing cells by plaque-forming cell (PFC) assays: the Cunningham-Szenberg technique and the Jerne-Nordin technique. The assay can be modified as described to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells. A protocol for preparing the resting B cells by Percoll gradient centrifugation is also described.