UNIT 17.6 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Zachara, N. E., Hart, G. W., Cole, R. N. and Gao, Y. 2002. Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine. Current Protocols in Molecular Biology. 57:II:17.6:17.6.1–17.6.25.
- Published Online: 1 FEB 2002
- Published Print: JAN 2002
This is not the most recent version of the article. View current version (1 JUL 2011)
The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.