Unit

UNIT 17.6 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine

  1. Natasha E. Zachara1,
  2. Gerald W. Hart1,
  3. Robert N. Cole1,
  4. Yuan Gao2

Published Online: 1 FEB 2002

DOI: 10.1002/0471142727.mb1706s57

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Zachara, N. E., Hart, G. W., Cole, R. N. and Gao, Y. 2002. Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine. Current Protocols in Molecular Biology. 57:II:17.6:17.6.1–17.6.25.

Author Information

  1. 1

    The Johns Hopkins University Medical School, Baltimore, Maryland

  2. 2

    Deakin University, Victoria, Australia

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: JAN 2002

This is not the most recent version of the article. View current version (1 JUL 2011)

Abstract

The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.