Unit

UNIT 17.6 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine

  1. Natasha E. Zachara1,
  2. Keith Vosseller2,
  3. Gerald W. Hart1

Published Online: 1 JUL 2011

DOI: 10.1002/0471142727.mb1706s95

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Zachara, N. E., Vosseller, K. and Hart, G. W. 2011. Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine. Current Protocols in Molecular Biology. 95:II:17.6:17.6.1–17.6.33.

Author Information

  1. 1

    The Johns Hopkins University School of Medicine, Baltimore, Maryland

  2. 2

    Drexel University College of Medicine, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 JUL 2011
  2. Published Print: JUL 2011

Abstract

O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites. Curr. Protoc. Mol. Biol. 95:17.6.1-17.6.33. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • signal transduction;
  • glycosylation;
  • O-GlcNAc;
  • method(s);
  • detection;
  • analysis;
  • galactosyltransferase;
  • O-linked;
  • glycosylation