UNIT 17.9 Direct Chemical Analysis of Glycoconjugates for Carbohydrates
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Manzi, A. and Esko, J. 2001. Direct Chemical Analysis of Glycoconjugates for Carbohydrates. Current Protocols in Molecular Biology. 32:II:17.9:17.9.1–17.9.11.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
This unit presents protocols for detection of different sugars bound to glycoconjugates. The solution containing the carbohydrate material is treated with a specific reagent, generating a colored reaction product that can be detected spectrophotometrically. For each assay, the absorbance of the colored solution is proportional to the amount of sugar present in the glycoconjugate.