Unit

UNIT 17.9 Direct Chemical Analysis of Glycoconjugates for Carbohydrates

  1. Adriana Manzi1,
  2. Jeffrey Esko2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1709s32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Manzi, A. and Esko, J. 2001. Direct Chemical Analysis of Glycoconjugates for Carbohydrates. Current Protocols in Molecular Biology. 32:II:17.9:17.9.1–17.9.11.

Author Information

  1. 1

    University of California San Diego, La Jolla, California

  2. 2

    University of Alabama at Birmingham, Birmingham, Alabama

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995

Abstract

This unit presents protocols for detection of different sugars bound to glycoconjugates. The solution containing the carbohydrate material is treated with a specific reagent, generating a colored reaction product that can be detected spectrophotometrically. For each assay, the absorbance of the colored solution is proportional to the amount of sugar present in the glycoconjugate.