UNIT 17.10B Inhibition of Glycolipid Biosynthesis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Manzi, A. E. 2001. Inhibition of Glycolipid Biosynthesis. Current Protocols in Molecular Biology. 32:II:17.10B:17.10.10–17.10.14.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
Adequate inhibition of glycolipid biosynthesis allows the study of their biological functions. The method presented in this unit employs a synthetic analog of ceramide, PDMP (1-phenyl-2-decanoylamino-3-morpholino-1-propanol), that inhibits glycolipid biosynthesis in cultured cells. Optimum conditions for inhibition of glycolipid biosynthesis are determined, glycolipids extracted from cultures grown with and without inhibitor, and the patterns of glycolipids analyzed by HPTLC. Detection is achieved using colorimetric reactions, or by monitoring radioactivity when cells have been metabolically radiolabeled.