UNIT 17.10B Inhibition of Glycolipid Biosynthesis

  1. Adriana E. Manzi

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1710bs32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Manzi, A. E. 2001. Inhibition of Glycolipid Biosynthesis. Current Protocols in Molecular Biology. 32:II:17.10B:17.10.10–17.10.14.

Author Information

  1. University of California, San Diego, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995


Adequate inhibition of glycolipid biosynthesis allows the study of their biological functions. The method presented in this unit employs a synthetic analog of ceramide, PDMP (1-phenyl-2-decanoylamino-3-morpholino-1-propanol), that inhibits glycolipid biosynthesis in cultured cells. Optimum conditions for inhibition of glycolipid biosynthesis are determined, glycolipids extracted from cultures grown with and without inhibitor, and the patterns of glycolipids analyzed by HPTLC. Detection is achieved using colorimetric reactions, or by monitoring radioactivity when cells have been metabolically radiolabeled.