UNIT 17.12 Sialidases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Powell, L. D. and Varki, A. P. 2001. Sialidases. Current Protocols in Molecular Biology. 27:III:17.12:17.12.1–17.12.8.
- Published Online: 1 MAY 2001
- Published Print: JUL 1994
Sialic acids are a family of nine-carbon acidic sugars found at the nonreducing terminus of many glycoconjugates. Sialidases can remove these sugar units selectively from cell surfaces, membranes, or purified glycoconjugates. In this unit, sialidase digestion of purified glycoproteins is described as is treatment of intact cells. The physical properties of the four most useful sialidases are discussed along with their relative activities against sialic acids with different modifications and in different linkages