UNIT 17.12 Sialidases

  1. Leland D. Powell,
  2. Ajit P. Varki

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1712s27

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Powell, L. D. and Varki, A. P. 2001. Sialidases. Current Protocols in Molecular Biology. 27:III:17.12:17.12.1–17.12.8.

Author Information

  1. University of California San Diego, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1994


Sialic acids are a family of nine-carbon acidic sugars found at the nonreducing terminus of many glycoconjugates. Sialidases can remove these sugar units selectively from cell surfaces, membranes, or purified glycoconjugates. In this unit, sialidase digestion of purified glycoproteins is described as is treatment of intact cells. The physical properties of the four most useful sialidases are discussed along with their relative activities against sialic acids with different modifications and in different linkages