UNIT 17.13A Endoglycosidase and Glycoamidase Release of N-Linked Glycans
Published Online: 1 JAN 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Freeze, H. H. and Kranz, C. 2010. Endoglycosidase and Glycoamidase Release of N-Linked Glycans. Current Protocols in Molecular Biology. 89:III:17.13A:17.13A.1–17.13A.25.
- Published Online: 1 JAN 2010
- Published Print: JAN 2010
Nearly all proteins entering the lumen of the endoplasmic reticulum (ER) become glycosylated en route to a cellular organelle, the plasma membrane, or the extracellular space. Many glycans can be attached to proteins, but the most common are the N-linked glycans (oligosaccharides). These chains are added very soon after a protein enters the ER, but they undergo extensive remodeling (processing), especially in the Golgi. Processing changes the sensitivity of the N-glycan to enzymes that cleave entire sugar chains or individual monosaccharides, which also changes the migration of the protein on SDS gels. These changes can be used to indicate when a protein has passed a particular subcellular location. This unit details some of the methods used to track a protein as it trafficks from the ER to the Golgi toward its final location. Curr. Protoc. Mol. Biol. 89:17.13A.1-17.13A.25. © 2010 by John Wiley & Sons, Inc.
- intracellular trafficking