UNIT 17.13B Analysis of Glycosaminoglycans with Polysaccharide Lyases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Linhardt, R. J. 2001. Analysis of Glycosaminoglycans with Polysaccharide Lyases. Current Protocols in Molecular Biology. 48:III:17.13B:17.13B.1–17.13B.16.
- Published Online: 1 MAY 2001
- Published Print: OCT 1999
Polysaccharide lyases are a class of enzymes useful for analysis of glycosaminoglycans (GAGs) and the glycosaminoglycan component of proteoglycans (PGs). These enzymes cleave specific glycosidic linkages present in acidic polysaccharides and result in depolymerization. The lyases are derived from a wide variety of pathogenic and nonpathogenic bacteria and fungi. This class of enzymes includes heparin lyases (heparinases), heparan sulfate lyases (heparanases or heparitinases), chondroitin lyases (chondroitinases), and hyaluronate lyases (hyaluronidases), all of which are described in this unit. Two protocols describe depolymerization of GAGs, and two support protocols describe assays to confirm and quantitate the activity of heparin and chondroitin ABC lyases.