Unit

UNIT 17.13B Analysis of Glycosaminoglycans with Polysaccharide Lyases

  1. Robert J. Linhardt

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1713bs48

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Linhardt, R. J. 2001. Analysis of Glycosaminoglycans with Polysaccharide Lyases. Current Protocols in Molecular Biology. 48:III:17.13B:17.13B.1–17.13B.16.

Author Information

  1. University of Iowa, Iowa City, Iowa

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1999

Abstract

Polysaccharide lyases are a class of enzymes useful for analysis of glycosaminoglycans (GAGs) and the glycosaminoglycan component of proteoglycans (PGs). These enzymes cleave specific glycosidic linkages present in acidic polysaccharides and result in depolymerization. The lyases are derived from a wide variety of pathogenic and nonpathogenic bacteria and fungi. This class of enzymes includes heparin lyases (heparinases), heparan sulfate lyases (heparanases or heparitinases), chondroitin lyases (chondroitinases), and hyaluronate lyases (hyaluronidases), all of which are described in this unit. Two protocols describe depolymerization of GAGs, and two support protocols describe assays to confirm and quantitate the activity of heparin and chondroitin ABC lyases.