Unit

UNIT 17.15A β-Elimination for Release of O-Linked Glycosaminoglycans from Proteoglycans

  1. H. Edward Conrad

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1715as31

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Conrad, H. E. 2001. β-Elimination for Release of O-Linked Glycosaminoglycans from Proteoglycans. Current Protocols in Molecular Biology. 31:III:17.15A:17.15.1–17.15.3.

Author Information

  1. University of Illinois, Urbana, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1995

Abstract

O-linked glycosaminoglycan (GAG) chains in proteoglycans are readily released from their core proteins by treatment with alkali at room temperature. This β-elimination is the same type of reaction as that for releasing O-linked oligosaccharides from their core proteins. Under the reaction conditions described here, N-linked oligosaccharides remain attached to the core protein, but any O-linked oligosaccharides will be released along with the GAG chains. The procedure can be used to isolate the free GAG chains, the free O-linked oligosaccharides, and the core protein (which will still have any N-linked oligosaccharides that were originally present).