UNIT 17.15A β-Elimination for Release of O-Linked Glycosaminoglycans from Proteoglycans
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Conrad, H. E. 2001. β-Elimination for Release of O-Linked Glycosaminoglycans from Proteoglycans. Current Protocols in Molecular Biology. 31:III:17.15A:17.15.1–17.15.3.
- Published Online: 1 MAY 2001
- Published Print: JUL 1995
O-linked glycosaminoglycan (GAG) chains in proteoglycans are readily released from their core proteins by treatment with alkali at room temperature. This β-elimination is the same type of reaction as that for releasing O-linked oligosaccharides from their core proteins. Under the reaction conditions described here, N-linked oligosaccharides remain attached to the core protein, but any O-linked oligosaccharides will be released along with the GAG chains. The procedure can be used to isolate the free GAG chains, the free O-linked oligosaccharides, and the core protein (which will still have any N-linked oligosaccharides that were originally present).