Unit

UNIT 17.15B β-Elimination for Release of O-GalNAc-Linked Oligosaccharides from Glycoproteins and Glycopeptides

  1. Minoru Fukuda

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1715bs31

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Fukuda, M. 2001. β-Elimination for Release of O-GalNAc-Linked Oligosaccharides from Glycoproteins and Glycopeptides. Current Protocols in Molecular Biology. 31:III:17.15B:17.15.4–17.15.9.

Author Information

  1. La Jolla Cancer Research Foundation, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1995

Abstract

This unit describes release of oligosaccharides that are attached to polypeptides through an N-acetylgalactosamine (GalNAc) linkage to the hydroxyl groups of serine or threonine. The b-elimination procedures described here can be used to recover the oligosaccharide chains (also called glycans) and/or identify the serine or threonine residues involved in the linkage. A b-elimination method employing sodium borohydride (NaBH4) and alkaline conditions is described, and an alternative method is also presented in which only alkaline conditions are used without a reducing agent. Another alternative protocol uses sodium sulfite.