UNIT 17.16 Acid Hydrolysis for Release of Monosaccharides
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Manzi, A. 2001. Acid Hydrolysis for Release of Monosaccharides. Current Protocols in Molecular Biology. 32:III:17.16:17.16.1–17.16.11.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
The first step in obtaining a compositional analysis of a glycoconjugate is the release of the individual monosaccharide constituents. Mild acid hydrolysis to release fucosyl residues from glycoconjugates is described in this unit, in addition to mild acid hydrolysis to release sialic acids from glycoconjugates, along with dialysis and column chromatography procedures to purify the sialic acids. Strong acid hydrolysis to release all monosaccharides from glycoconjugates is also detailed.