Unit

UNIT 17.16 Acid Hydrolysis for Release of Monosaccharides

  1. Adriana Manzi

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1716s32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Manzi, A. 2001. Acid Hydrolysis for Release of Monosaccharides. Current Protocols in Molecular Biology. 32:III:17.16:17.16.1–17.16.11.

Author Information

  1. University of California San Diego, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995

Abstract

The first step in obtaining a compositional analysis of a glycoconjugate is the release of the individual monosaccharide constituents. Mild acid hydrolysis to release fucosyl residues from glycoconjugates is described in this unit, in addition to mild acid hydrolysis to release sialic acids from glycoconjugates, along with dialysis and column chromatography procedures to purify the sialic acids. Strong acid hydrolysis to release all monosaccharides from glycoconjugates is also detailed.