UNIT 17.17A Enzymatic Release of Oligosaccharides from Glycolipids
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Manzi, A. E. 2001. Enzymatic Release of Oligosaccharides from Glycolipids. Current Protocols in Molecular Biology. 32:III:17.17A:17.17.1–17.17.5.
- Published Online: 1 MAY 2001
- Published Print: OCT 1995
Selective cleavage of the oligosaccharide moiety of glycolipids for further structural analysis can be achieved by means of endoglycoceramidase (EGCase), an enzyme specific for the linkages between oligosaccharide and ceramide residues in glycolipids. The method described here can be used for analysis of glycolipids present in macroscopic amounts or for glycolipids that have been radiolabeled in their carbohydrate moiety.