UNIT 17.17A Enzymatic Release of Oligosaccharides from Glycolipids

  1. Adriana E. Manzi

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb1717as32

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Manzi, A. E. 2001. Enzymatic Release of Oligosaccharides from Glycolipids. Current Protocols in Molecular Biology. 32:III:17.17A:17.17.1–17.17.5.

Author Information

  1. University of California San Diego, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1995


Selective cleavage of the oligosaccharide moiety of glycolipids for further structural analysis can be achieved by means of endoglycoceramidase (EGCase), an enzyme specific for the linkages between oligosaccharide and ceramide residues in glycolipids. The method described here can be used for analysis of glycolipids present in macroscopic amounts or for glycolipids that have been radiolabeled in their carbohydrate moiety.